"Synthetic Biology: a new approach to bioenergy technology"
- ■日 時 2017/10/05（木）15：00-16：30
- ■場 所 エコラボ 第4講義室
- ■講 演 張瑞仁先生 台湾中国医薬大学 医学研究所 副研究員
Abstract Cellulose is a renewable feedstock for green industry, and it is impor-
tant to develop a technique to construct a host with a high cellulolytic efficiency to
digest cellulose. In this study, we developed a convenient host-engineering tech-
nique to adjust the expression levels of heterologous genes in the host by promoter rearrangement and
gene copy number adjustment. Using genes from different glycoside hydrolase (GH) families including
GH2, GH3, GH5, GH6, GH7, and GH12 from three cellulolytic fungi, we constructed a cellulolytic yeast
Kluyveromyces marxianus with 8 cellulase gene-cassettes that produced a cellulase cocktail with a high
cellulolytic efficiency and it showed a significant reduction in enzyme cost in a rice straw saccharification
process. In general, our technique can be used to design a host that can efficiently convert biomass
feedstock to biofuel.
Dr. Jui-Jen Chang
EDUCATION: B.S. Continuing Education Credit Courses, Multi-Discipline Management of Technology, National
Chengchi University, Taiwan (2014/3-2014/6) / Ph.D., Department of Life Sciences, National Chung Hsing University,
Taiwan (2001-2006) / B.S., Department of Nutrition, Chung Shan Medical University, Taiwan (1997-2001)
EXPERTISE: Synthetic biology, Biochemistry, Microbiology, Molecular biology, Biopharmaceuticals
STUDY FIELDS: Synthetic biology and its applications, Biofuel and environmental biotechnology, Designer cell
factory for production of functional metabolites and biopharmaceuticals.
※ The lecture will be given in English.
環境修復生態学分野 簡梅芳 助教